Regulatory

Part:BBa_K1104203:Design

Designed by: Ting-Yun Chiang   Group: iGEM13_NYMU-Taipei   (2013-09-16)


AhpCp1000


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Primers For PCR

AhpCp1000_FP gcttctagag tccatcagtttctcattgtcacttaacact
AhpCp1000_RP gga ctgcagcggccgctactagta ctatacttcctccgtgttttcgatgaga
ahpC promoter(K362001)has a Pst1 site in the sequence.
We designed this part AhpC(mutated), and succesfully mutated the Pst1 site (ctgcag->ctacag).
ahpCp_mut_FP cacctcagcctacaggcaggcactgaa
ahpCp_mut_RP ttcagtgcctgcctgtaggctgaggtg

PCR Time Protocol

Temperature(°C) Time(sec) Number of Cycles
94 120 --
94 15 35
53 30
72 68
72 300 --

Source

E. coli MG1655 genomic sequence

References